The three things that matter in photography of edges

[grepper]

Good point Mr. Larrin.

I called AmScope on the phone and got some guy who didn’t impress me as particularly knowledgeable.  I asked him if the 100X objective required oil immersion and he said no, but I could tell he was just looking at the printed specs.
 
Oddly it does not specify oil for the 100X objective on the AmScope website for that scope, but it sure does on Amazon.  Additionally, here https://www.amscope.com/how-to-use-microscope on the AmScope website they state, “Use the 100X objective with extreme care. As it is an oil immersion lens…”.
 
So, that sucks.  However the 40X objective with the 16X eyepieces would be 640X.  Still not bad, but if that is the case I’d want to check around to see what else is available. 
 
I don’t know what any of that means for the camera.  Does the camera only see the objective magnification?  What is the max optical magnification for the camera?
 
Also, an inverted scope might be handy for larger specimens such as looking at a cleaver edge-on.  Of course large specimens are simply not an issue for hand-held scopes like the mighty little Dino-Lite.

[Larrin]

I don’t believe the camera has any way to optically zoom. I’ve never adjusted anything like that in any microscope I’ve used.

[Larrin]

Maybe something like this would do the trick: https://www.amazon.com/dp/B014AZT1TQ/ref...il_0?psc=1

[grepper]

Mr. Larrin, I have no experience with cameras on microscopes.  It sounds like maybe you do, so any info you wish to share is helpful.
 
Apparently the cameras on the AmScopes have 40X magnification so they have reduction lenses to knock them back to 20X.  I take it that means when using a 40X objective the camera would see 800X.  Is that correct?
 
I don’t really understand the camera having 40X magnification because they show when connecting a reduction lens there is no lens in front of the camera sensor.  They also show that a camera can be attached by removing an eyepiece. But that would remove the eyepiece lens and the magnification it provides. Now that I think the thing through, I have no idea how cameras attached to microscopes work. Obviously the camera uses the objective lens magnification but is there additional magnification somehow? I guess I don’t get it.
 
The AmScope cameras can be focused, but not zoomed.
 
Here is a video.  Starting about ¾ through it speaks to reduction lenses and how they attach.
 
https://www.youtube.com/watch?v=19HwRyA5...e=youtu.be
 
I would like to understand what magnification the camera sees.

[Larrin]

You’ve reached the limit of my knowledge. I’ve used several high end microscopes but I didn’t own any of them because they were all University microscopes. We didn’t adjust the magnification of the camera on any of them. If that was a feature available it was not one we used.

[EOU]

Hello Cyrano. Another great bunch of pictures indicative of the capabilities of your microscope. Image stacking seems as if it might provide a window looking onto what has previously remained unseen. Do you think that rolled edges might be able to be seen with a scope like or similar to the one that you use? We're speaking of the sort of edge that might begin at 150 and then roll to 350-450. In order to give our question to you a little more perspective; often, edges that have rolled 250 or 300 points can be detected by hooking a fingernail on the rolled section of the edge. Additionally, a roll of this magnitude can usually be seen with the naked eye when overhead light is reflected off of it. It would seem that a defect in the edge of this physical size should be able to be seen if the DOF/lighting issues could be resolved. As it is, we have never been able to capture any of this using our standard digital microscopy equipment. When we say "standard" we mean cheap.

[Cyrano]

...  Do you think that rolled edges might be able to be seen with a scope like or similar to the one that you use? ...

If you have a sample on hand which you can send to me, I'll be happy to attempt to image it.

[EOU]

You know Cyrano we think we'll just take you up on that generous offer. Fortunately, with a SET unit it doesn't take long to generate a sample. If you'd be kind enough to email us at our general email (edgeonup@gmail.com) with an address, we'll head a rolled edge your direction.

[Cyrano]

You know Cyrano we think we'll just take you up on that generous offer. Fortunately, with a SET unit it doesn't take long to generate a sample. If you'd be kind enough to email us at our general email (edgeonup@gmail.com) with an address, we'll head a rolled edge your direction.

Done!

[EOU]

Yes it is done or, at least, about to be done. We just need to get the edges to Cyrano.